Our webinterface 5.4 on Windows server 2008 R2 was slow as hell for the first time load for any user or just after a reboot. Be sure to check your ASPNET version and path first.

Edit the ASPNET.config file Path: “c:WindowsMicrosoft.NETFrameworkv2.0.50727Aspnet.config“ between the

<generatePublisherEvidence enabled=”false”/>

 Example

<?xml version=”1.0″ encoding=”UTF-8″ ?>
<configuration>
    <runtime>
        <legacyUnhandledExceptionPolicy enabled=”false” />
        <legacyImpersonationPolicy enabled=”true”/>
        <alwaysFlowImpersonationPolicy enabled=”false”/>
        <SymbolReadingPolicy enabled=”1″ />
 <generatePublisherEvidence enabled=”false”/>
    </runtime>
</configuration>

REFERENCES: http://support.citrix.com/article/CTX117273